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Fig. 8. TMBIM1 knockdown suppresses autophagy and EMT in an intracranial xenograft model. (A) In vivo bioluminescence imaging of nude mice 30 days after cell inoculation. (B) Mouse survival was shown by Kaplan‒Meier analysis. The log-rank test was used to measure survival differences. (C) HE staining. (D) IHC staining of p-AMPK (Thr172), <t>p-ULK1</t> <t>(Ser317),</t> p-mTOR (Ser2448), Beclin1, P62 and TMBIM1. Scale bars, 20 um. (E) IHC staining of E-cadherin, N-cadherin, Vimentin and SNAI1. Scale bars, 20 um.
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Fig. 8. TMBIM1 knockdown suppresses autophagy and EMT in an intracranial xenograft model. (A) In vivo bioluminescence imaging of nude mice 30 days after cell inoculation. (B) Mouse survival was shown by Kaplan‒Meier analysis. The log-rank test was used to measure survival differences. (C) HE staining. (D) IHC staining of p-AMPK (Thr172), <t>p-ULK1</t> <t>(Ser317),</t> p-mTOR (Ser2448), Beclin1, P62 and TMBIM1. Scale bars, 20 um. (E) IHC staining of E-cadherin, N-cadherin, Vimentin and SNAI1. Scale bars, 20 um.
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Fig. 8. TMBIM1 knockdown suppresses autophagy and EMT in an intracranial xenograft model. (A) In vivo bioluminescence imaging of nude mice 30 days after cell inoculation. (B) Mouse survival was shown by Kaplan‒Meier analysis. The log-rank test was used to measure survival differences. (C) HE staining. (D) IHC staining of p-AMPK (Thr172), p-ULK1 (Ser317), p-mTOR (Ser2448), Beclin1, P62 and TMBIM1. Scale bars, 20 um. (E) IHC staining of E-cadherin, N-cadherin, Vimentin and SNAI1. Scale bars, 20 um.

Journal: Scientific reports

Article Title: TMBIM1 promotes epithelial mesenchymal transition by accelerating autophagic degradation of E-cadherin in glioblastoma.

doi: 10.1038/s41598-025-01699-4

Figure Lengend Snippet: Fig. 8. TMBIM1 knockdown suppresses autophagy and EMT in an intracranial xenograft model. (A) In vivo bioluminescence imaging of nude mice 30 days after cell inoculation. (B) Mouse survival was shown by Kaplan‒Meier analysis. The log-rank test was used to measure survival differences. (C) HE staining. (D) IHC staining of p-AMPK (Thr172), p-ULK1 (Ser317), p-mTOR (Ser2448), Beclin1, P62 and TMBIM1. Scale bars, 20 um. (E) IHC staining of E-cadherin, N-cadherin, Vimentin and SNAI1. Scale bars, 20 um.

Article Snippet: Anti-phospho-AMPKα (Thr172) (#2535), anti-phospho-ULK1 (Ser317) (#12753) and anti-phospho-mTOR (Ser2448) (#5536) antibodies were obtained from Cell Signaling Technology (USA).

Techniques: Knockdown, In Vivo, Imaging, Staining, Immunohistochemistry